Proinsulin Intermolecular Interactions during Secretory Trafficking in Pancreatic β Cells
نویسندگان
چکیده
منابع مشابه
PERK (EIF2AK3) Regulates Proinsulin Trafficking and Quality Control in the Secretory Pathway
OBJECTIVE Loss-of-function mutations in Perk (EIF2AK3) result in permanent neonatal diabetes in humans (Wolcott-Rallison Syndrome) and mice. Previously, we found that diabetes associated with Perk deficiency resulted from insufficient proliferation of beta-cells and from defects in insulin secretion. A substantial fraction of PERK-deficient beta-cells display a highly abnormal cellular phenotyp...
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By quantitative immunoelectron microscopy and HPLC, we have studied the effect of disrupting pH gradients, by ammonium chloride, on proinsulin conversion in the insulin-producing B-cells of the islets of Langerhans. Proinsulin content and pH in single secretory vesicles were measured on consecutive serial sections immunostained alternately with antiproinsulin or anti-dinitrophenol (to reveal th...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2013
ISSN: 0021-9258
DOI: 10.1074/jbc.m112.420018